12/19/2023 0 Comments Bluex lab![]() ![]() It has been developped in the laboratories of research of Doceram GmbH in Dortmund and now is in process to obtain the CE approval. This diagnostic DNA test can detect all three known variants of the MLPH gene.This exceptional product will be available on the market only in autumn. Dogs that are compound heterozygotes d 1/d 2 , d 2/d 3 , or d 1/d 3 have a dilute phenotype. The phenotype produced by two copies of the d 3 variant is unknown, but this mutation is predicted to shorten the MLPH protein and likely disrupt function. The effect of the d 2 mutation on the resultant protein is unknown, although in the homozygous state it dilutes color. ![]() The d 1 mutation is known to cause a reduction in the amount of full-length protein product, thus producing less pigment. Two copies of any of the three dilution variants, or any combination of two of these variants, are necessary to lighten the color. The d 3 variant has been detected in the Italian Greyhound, Chihuahua, Mudi, Pumi, Shih Tzu, Pekingese, Tibetan Mastiff, Yorkshire Terrier, Shetland Sheepdog, indigenous dogs, and wolf-dog hybrids (Van Buren et al. Subsequent research at the VGL identified a third, very rare dilution variant d 3 (c.667_668insC, p.His223Profs*41). Analysis at the Veterinary Genetics Laboratory (VGL) determined that the d 2 variant is present at a frequency of less than 1% in phenotypically dilute French Bulldogs, thus suggesting that additional variants also cause this phenotype. identified a second dilution in MLPH, variant d 2 (c.705G>C, p.Gln235His) in the Sloughi, Chow Chow, and Thai Ridgeback dogs. However, this mutation alone does not account for all dilute color phenotypes. Results of this test can be submitted to the OFA (Orthopedic Foundation for Animals)Ĭhocolate/liver diluted to Silver Gray in a Weimaraner, a breed fixed for diluteĪ recessive mutation d 1, previously named d, in the melanophilin ( MLPH) gene (g.48121642G>A, c.-22G>A) was identified as the cause of color dilution phenotypes in several dog breeds (Drögemüller et al. ![]() ![]() They will transmit a dilution variant to all of their offspring. Dogs with d 1/d 1, d 2/d 2, d 3/d 3, d 1/d 2, d 1/d 3, or d 2/d 3 genotype are expected to display dilute coat colors.Matings between two carriers of dilute variants are expected to produce 25% puppies with dilute coat colors. They will transmit the dilution variant they carry to 50% of their offspring. Dogs with D/d 1, D/d 2, or D/d 3 genotype are not expected to display dilute coat colors, but they are carriers of dilution variants.They cannot produce dilute offspring or transmit any of the known dilution variants to their offspring. Dogs with D/D genotype are not expected to display dilute coat colors they have no known dilution variants.For example, black becomes a gray-blue color (often called "blue" by breeders) and chocolate brown becomes a pale silvery red (in some breeds, this can be called "lilac" in the French Bulldog, this is typically referred to as "isabella" - other breeds may have different names for this dilution).Īlleles: D = Non-dilute, d 1 = Dilute (variant 1, common), d 2 = Dilute (variant 2), d 3 = Dilute (variant 3, rare)īreeds appropriate for testing: Many breeds Phenotype: Base coat colors are lightened (diluted) to paler shades. Black coloration diluted to blue in an American Staffordshire Terrier ![]()
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